Investigation of aminoglycoside modifying enzyme genes in methicillin-resistant staphylococci

Ardic N., SAREYYÜPOĞLU B., Ozyurt M., Haznedaroglu T., Ilga U.

MICROBIOLOGICAL RESEARCH, vol.161, no.1, pp.49-54, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 161 Issue: 1
  • Publication Date: 2006
  • Doi Number: 10.1016/j.micres.2005.05.002
  • Journal Name: MICROBIOLOGICAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.49-54
  • Keywords: MRSA, MRCNS, aminoglycoside resistance, mecA, aminoglycoside modifying enzyme, ANTIBIOTIC-RESISTANCE, AUREUS, EPIDEMIOLOGY
  • Ankara University Affiliated: Yes

Abstract

Methicillin-resistant staphylococci may also be resistant to some other antibiotics as well as beta-lactams. In this study, co-existence of resistance to methicillin and aminoglycosides was genetically investigated in staphylococci. A total of 50 staphylococci from in-patients, 17 Staphylococcus aureus and 33 coagulase negative staphylococci (CNS) that contained mecA (gene encoding PBP 2a, an altered penicillin-binding protein) determined by polymerase chain reaction (PCR) were included in the study. Aminoglycoside modifying enzyme (AME) genes were investigated using multiplex-PCR. Aminocyclitol-6'-acetyltransferase-aminocyclitol-2"-phosphotransferase [aac(6')/aph(2")] gene (encoding bifunctional acetyltransferases/phosphotransferases) was determined in 66% of the isolates, aminocyclitol-4'-adenylytransferase (ant(4')-Ia) gene (encoding phosphotransferases) in 24%, and aminocyclitol-3'-phosphotransferase (aph(3')-IIIa) gene (encoding nucleotidyltransferases) in 8%. Two isolates contained all these three genes. Thirty-six (72%) isolates had at least one of these genes. Three CNS and one S. aureus isolates sensitive to oxacillin had the mecA gene. In conclusion, a high rate of aminoglycoside resistance was determined in methicillin-resistant staphylococci. The aac(6')/aph(2") was the most frequently detected. (c) 2005 Elsevier GmbH. All rights reserved.