Investigation of methicillin resistance and aminoglycoside modifying enzyme genes in hospital staphylococci by multiplex-polymerase chain reaction Hastane izolatι stafilokoklarda metisilin direnç ve aminoglikozid modifiye edici enzim genlerinin multipleks-polimeraz zincir reaksiyonu ile araştιrιlmasι

Özyurt M., SAREYYÜPOĞLU B., Ardiç N., Ilga U., Erdemoǧlu A., Haznedaroǧlu T.

Mikrobiyoloji Bulteni, vol.39, no.1, pp.1-8, 2005 (Scopus, TRDizin) identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 1
  • Publication Date: 2005
  • Journal Name: Mikrobiyoloji Bulteni
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1-8
  • Keywords: Aminoglycoside modifying enzyme, Methicilin, Multiplex-polymerase chain reaction, Staphylococci
  • Ankara University Affiliated: Yes

Abstract

This study was conducted to investigate the presence of methicillin and aminoglycoside resistance encoding genes by multiplex-polymerase chain reaction (PCR) and by phenotypic methods in staphylococci isolated from inpatients' clinical specimens. The presence of aac(6′)Iaph(2″), aph(3′)-IIIa and ant(4′)-Ia genes encoding aminoglycoside modifying enzymes (AME) and mecA gene encoding methicillin resistance were genotypically investigated. A total of 19 S. aureus and 30 coagulase negative staphylococci (CNS) were tested. Thirty four (69.4%) of the isolates were found to be resistant to oxacillin with disk diffusion test, 33 (97%) of which were found to harbour mecA gene. The correspondance between oxacillin resistance and presence of mecA gene was found to be 100% in S. aureus isolates, while it was 95.7% in CNS. Twenty two (44.9%), 7 (14.3%) and 2 (4.1%) isolates were found to harbour aac(6′)/aph(2″)Iaph(3′)-IIIa and ant(4′)- Ia AME genes, respectively. At least one or more AME genes were detected in 72.7% of mecA positive isolates.