Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters

Bucak M. N., Keskin N., Taşpınar M., Coyan K., Başpınar N., Cenariu M. C., ...More

CRYOBIOLOGY, vol.67, no.1, pp.34-39, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 1
  • Publication Date: 2013
  • Doi Number: 10.1016/j.cryobiol.2013.04.007
  • Journal Name: CRYOBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.34-39
  • Keywords: Ram sperm, Additives, Motility, Flourescent staining parameters, Comet test, TUNEL-positive cells, OXIDATIVE STRESS PARAMETERS, LIPID-PEROXIDATION, SUPEROXIDE-DISMUTASE, EGG-YOLK, GLUTATHIONE-PEROXIDASE, FERTILIZING ABILITY, MOUSE SPERMATOZOA, DNA FRAGMENTATION, BOVINE SPERM, SEMEN
  • Ankara University Affiliated: Yes

Abstract

The aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze-thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10 mM raffinose, 5 mM hypotaurine, 5 mM raffinose +2.5 mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 degrees C for 25 s in a water bath for evaluation.