Electrochemical study of S-adenosyl-L-methionine and its differential pulse and square-wave voltammetric determination

USLU B., ÖZKAN S. A., Aboul-Enein H. Y.

Electroanalysis, vol.14, no.11, pp.736-740, 2002 (SCI-Expanded) identifier

  • Publication Type: Article / Review
  • Volume: 14 Issue: 11
  • Publication Date: 2002
  • Journal Name: Electroanalysis
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.736-740
  • Keywords: Differential pulse voltammetry, Pharmaceutical preparations, S-Adenosyl-L-methionine (SAM), Square-wave voltammetry
  • Ankara University Affiliated: Yes

Abstract

The electrochemical oxidation of S-adenosyl-L-methionine (SAM) has been carried out in aqueous solution in the pH range 1.5-11.0 by cyclic, linear sweep, differential pulse (DPV) and square-wave (SWV)voltammetry. SAM exhibits one oxidation peak or wave depending on pH. The oxidation process was shown to be irreversible. For analytical purposes, a very well resolved diffusion controlled voltammetric peak was obtained in phosphate buffer at pH 2.04 for DPV and SWV techniques. The linear response was obtained in the ranges of 2 × 10-5-6 × 10-4 M for both techniques with a detection limit of 2.6 × 10-6 M for DPV and 2.4 × 10-6 M for SWV. Based on this study, simple, rapid and selective two voltammetric methods were developed for the determination of SAM in tablet dosage forms.