Quantification of shikimic acid in the methanolic extracts of three Alnus taxons growing in Turkey Türkiye’de yetişen üç Alnus taksonunun metanollü ekstrelerinde şikimik asit miktar tayini

Altinyay Ç., ERGENE B., ALTUN M. L.

Turkish Journal of Pharmaceutical Sciences, vol.13, no.1, pp.115-120, 2016 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.5505/tjps.2016.29591
  • Journal Name: Turkish Journal of Pharmaceutical Sciences
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.115-120
  • Keywords: Alnus, Betulaceae, HPLC, Quantitative analysis, Shikimic acid
  • Ankara University Affiliated: Yes

Abstract

© 2016, Turkish Pharmacists Association. All rights reserved.Shikimic acid is a phenolic acid which is known to possess several activities and it takes attention as a leading compound to some synthetic medicinal substances as well. In this study, high performance liquid chromatographic analyses were carried out in order to determine shikimic acid contents of Alnus glutinosa subsp. glutinosa, A. orientalis var. orientalis and A. orientalis var. pubescens; acetonitrile and 0.2% o-phosphoric acid:water mixture with isocratic flow was used as mobile phase. The flow rate was 0.5 ml/min. The quantitative analysis of methanol extracts prepared from the leaves of A. glutinosa subsp. glutinosa, A. orientalis var. orientalis and A. orientalis var. pubescens revealed that shikimic acid amount was found to be 0.6491%, 0.4309% and 0.2452% respectively.