Evaluation of the antioxidant effects of some flavonylthiazolidinediones by determining their effects on lipid peroxidation, superoxide anion formation, and 2,2-diphenyl-1-picrylhydrazyl stable free radical

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Tuncbilek M., EKE B., KILCIGİL G., Coban T., Iscan M.

Biological and Pharmaceutical Bulletin, vol.27, no.6, pp.912-915, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 6
  • Publication Date: 2004
  • Doi Number: 10.1248/bpb.27.912
  • Journal Name: Biological and Pharmaceutical Bulletin
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.912-915
  • Keywords: antioxidant activity, flavonylthiazolidinedione, lipid peroxidation, superoxide dismutase, 2,2-diphenyl-1-picryl-hydrazyl (DPPH), FLAVONOIDS, DERIVATIVES, TROGLITAZONE, MECHANISMS
  • Ankara University Affiliated: Yes

Abstract

The in vitro antioxidant effects of some flavonylthiazolidinediones (Ia-d, IIa-d) on rat liver microsomal NADPH-dependent lipid peroxidation (LP) levels were determined by measuring the formation of 2-thiobarbituric acid reactive substance. The free radical-scavenging properties of the compounds were examined in vitro by determining the capacity to scavenge superoxide anion formation and of the interaction with the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The compounds had no inhibitory effects on LP. However, they had variable inhibitory influence on superoxide anion production and DPPH radical in a concentration-dependent manner. The most active compound, 3-(2,5-dimethoxyphenacyl)-5-[2-phenyl-4H-4-oxo-1-benzopyran-6-yl)-methylenyl] -thiazolidine-2,4-dione, Id showed 98% inhibition of superoxide anion production and 95% inhibition of DPPH stable free radical at 10-3 M. © 2004 Pharmaceutical Society of Japan.