Genotoxic and Genoprotective Potential of Black Mulberry (Monts nigra) Fruit

YILMAZ S., UÇAR A., GÖKTAŞ B.

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, vol.91, no.4, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 91 Issue: 4
  • Publication Date: 2019
  • Doi Number: 10.1590/0001-3765201920190337
  • Journal Name: ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Black mulberry, chromosomal aberrations, micronucleus, genotoxicity, anti-genotoxicity, MORUS-NIGRA, MITOMYCIN-C, ANTIOXIDANT PROPERTIES, CHEMICAL-PROPERTIES, BIOACTIVE COMPOUNDS, FLAVONOID CONTENTS, MEDICINAL-PLANTS, EXTRACT, RUBRA, L.
  • Ankara University Affiliated: Yes

Abstract

Black Mulberry (Morus nigra L.) belongs to Moraceae family. The present study evaluated the possible genotoxic and/or protective activities of black mulbeny fruit juice (BMFJ), in vitro, using mitomycin C (MMC) as a positive control, by chromosomal aberrations and micronucleus assays. Human lymphocytes were treated with BMFJ concentrations alone (1/1, 1/2, 1/4, 1/8 dilutions), pretreatment (49h) (0.20 mu g/ml MMC+ 1/1 BMFJ, 0.20 mu g/ml MMC+1/2 diluted BMFJ, 0.20 mu g/ml MMC+1/4 diluted BMFJ, 0.20 mu g/ml MMC+1/8 diluted BMFJ) and simultaneous-treatment (48h) (0.20 mu g/ml MMC+ 1/1 BMFJ. 0.20 mu g/ml MMC+1/2 diluted BMFJ. 0.20 mu g/ml MMC+1/4 diluted BMFJ, 0.20 mu g/ml MMC+1/8 diluted BMFJ). The in vitro results demonstrated that BMFJ showed no genotoxicity, but it significantly decreased chromosomal aberration and micronucleus frequency induced by MMC. Our results showed that all concentrations of BMFJ revealed no genotoxicity but protective activity against genomic changes induced by anti-tumor agent MMC in human lymphocytes. Protective effects of BMFJ on MMC induced chromosomal damages most probably due to its free radical scavenging activity.