Three-dimensional (3D) cell culture studies: a review of the field of toxicology

İPEK S., ÜSTÜNDAĞ A., Can Eke B.

DRUG AND CHEMICAL TOXICOLOGY, cilt.46, sa.3, ss.523-533, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 46 Sayı: 3
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/01480545.2022.2066114
  • Dergi Adı: DRUG AND CHEMICAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.523-533
  • Anahtar Kelimeler: 2D cell culture, traditional cell culture, monolayer cell culture, 3D cell culture, 3D cell culture models, organ toxicity, toxicology, REPRODUCTIVE TOXICITY, COCULTURE MODEL, HYDROGELS, TISSUES
  • Ankara Üniversitesi Adresli: Evet

Özet

Traditional two-dimensional (2D) cell culture employed for centuries is extensively used in toxicological studies. There is no doubt that 2D cell culture has made significant contributions to toxicology. However, in today's world, it is necessary to develop more physiologically relevant models. Three-dimensional (3D) cell culture, which can recapitulate the cell's microenvironment, is, therefore, a more realistic model compared to traditional cell culture. In toxicology, 3D cell culture models are a powerful tool for studying different tissues and organs in similar environments and behave as if they are in in vivo conditions. In this review, we aimed to present 3D cell culture models that have been used in different organ toxicity studies. We reported the results and interpretations obtained from these studies. We aimed to highlight 3D models as the future of cell culture by reviewing 3D models used in different organ toxicity studies.