Detection of Enterohemolysin and Intimin Genes in Escherichia coli O157:H7 Strains Isolated from Calves and Cattle in Afyonkarahisar - Turkey

KUYUCUOĞLU Y., ŞEKER E., SAREYYÜPOĞLU B., GÜRLER Z.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.17, no.4, pp.663-666, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 4
  • Publication Date: 2011
  • Journal Name: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.663-666
  • Keywords: Calves, Cattle, eaeA, EhlyA, Escherichia coli O157:H7, PCR, TOXIN, ASSOCIATION, CONSUMPTION, HEMOLYSIN, OUTBREAKS, O157H7, EAE
  • Ankara University Affiliated: Yes

Abstract

The aim of this study was to detect enterohemolysin (EhlyA) and intimin (eaeA) virulence genes of 14 Escherichia coli O157:H7 strains isolated from 457 fecal samples (237 calves and 220 cattle) by PCR. While EhlyA gene was determined in 13 (92.8%) strains, the eaeA gene was positive in 8 (57.1%) strains. Of the 8 eaeA genes, 4 (50.0%) were obtained from diarrheic calves, 2 (25.0%) from non-diarrheic calves, and 2 (25.0%) from healthy cattle. A total of 7 (50.0%) strains were determined to harbour both of the EhlyA and eaeA genes. This study confirmed that calves, especially diarrheic, and cattle are a reservoir of E. coli O157:H7 strains that may be pathogenic for human.