Effect of 7-dehydrocholesterol and Cholesterol-loaded Cyclodextrins on Bull Sperm Motility During Short Term Storage


İNANÇ M. E., TEKİN K., OLĞAÇ K. T., ÖZEN D., Stelletta C., UYSAL O., ...More

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.23, no.4, pp.661-664, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.9775/kvfd.2017.17181
  • Journal Name: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.661-664
  • Keywords: Bull sperm, Cholesterol, Motility, Short term storage, 7-dehydrocholesterol, LECITHIN-BASED EXTENDER, CRYOSURVIVAL, QUALITY
  • Ankara University Affiliated: Yes

Abstract

The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4 degrees C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred and stored at +4 degrees C and sperm motility was analysed in determined intervals during three days storage period with computer aided sperm analyse (CASA) system. Motility decreased in all groups; however, 7-DHCLC and CLC groups motility decreased in the next two days gradually. But, CLC 1.5 mg/120x10(6) group were identified as the best groups for protecting motility in short time preservation. In conclusion, adding different rates of 7-DHCLC and CLC to semen extender preserved motility for three days in bull semen stored at +4 degrees C.