Turkish Journal of Pharmaceutical Sciences, vol.10, no.2, pp.255-262, 2013 (SCI-Expanded)
The aim of this study was to develop a reversed-phase high performance liquid chromatographic (RP- HPLC) method for the simultaneous determination of quinapril (QNP) and hydrochlorothiazide (HCZ) in pharmaceutical dosage forms. In this method quinapril, hydrochlorothiazide and perindopril (internal standard) were separated using a reversed phase column (Hichrom C18; 250×4.6 mm Ldfl0 μm) with acetonitrile: potassium dihydrogen phosphate (at pH 2.5 0.067 M) (40:60 v/v) as a mobile phase. UV visible dedector set at 211 nm and mobile phase was pumped at 1 0 mL/min flow rate The chromatographic separation was performed at 25 oC In these conditions the retention times for quinapril hydrochlorothiazide and perindoprile were 4 391 3 237 and 3 931 min respectively Linearity was obtained in the concentration range of 2-30 ug/mL. for quinapril and 1 25-18 75 μg/mL for hydrochlorothiazide The proposed method has been Illy validated and allows a number of cost-and Le-savinz benefits It was successfully applied to the determination of QNP and HCZ in synthetic mixtures and in pharmaceutical dosage forms. The proposed method is simple, rapid and suitable for quality control (QC) applications.