Research Information System
Journal of Liquid Chromatography and Related Technologies, vol.25, no.9, pp.1447-1456, 2002 (SCI-Expanded)
In the present study, a simple, sensitive, precise, and rapid HPLC method with UV detection for the analysis of lamivudine is developed and applied to the determination of anti HIV drug in commercial pharmaceutical dosage forms (tablets and oral solutions) and human serum. The gradient elution is performed with methanol: water (75:25, v/v) at a flow rate of 0.8 mL · min-1, using a Spherisorb® C18 analytical column 150 x 4.6 mm, 5 μm. Deflazacort is used as internal standard. Absorbance is monitored at 265 nm. Total analysis time was ∼6 min. Data, with respect to precision and accuracy and limits of detection, are reported and discussed. The described method can be readily utilized for analysis of pharmaceutical products and pharmacokinetic studies as well.