Stimuli-responsive properties of aminophenylboronic acid-carrying thermosensitive copolymers

Uguzdogan E., KAYI H., Denkbas E., Patir S., Tuncel A.

POLYMER INTERNATIONAL, vol.52, no.5, pp.649-657, 2003 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 52 Issue: 5
  • Publication Date: 2003
  • Doi Number: 10.1002/pi.1169
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.649-657
  • Keywords: thermosensitive polymer, pH sensitivity, N-isopropylacrylamide, aminophenylboronic acid, RNA, DNA, boronate affinity chromatography, CRITICAL SOLUTION TEMPERATURE, ALPHA-CHYMOTRYPSIN, GRAFT-COPOLYMERS, GEL BEADS, AFFINITY-CHROMATOGRAPHY, N-ISOPROPYLACRYLAMIDE, NUCLEOTIDE ADSORPTION, AQUEOUS-SOLUTION, SIDE-CHAINS, PH
  • Ankara University Affiliated: No


Thermosensitive copolymers of N-isopropylacrylamide (NIPA) and N-acryloxysuccinimide (NASI) were obtained by solution polymerization using azobisisobutyronitrile as the initiator in a tetrahydrofuran-toluene mixture at 65degreesC. A boronic acid-carrying ligand, m-aminophenylboronic acid (APBA) was covalently attached to the thermosensitive copolymer via the reaction between amino and succinimide groups. APBA-coupled thermosensitive copolymer exhibited both temperature and pH sensitivity. Thermally reversible phase transitions were observed both in the acidic and alkaline pH region for the APBA-modified copolymers obtained with different NASI feed concentrations. In our study, ribonucleic acid (RNA) was selected as a biomolecule having reactive groups which could potentially interact with the boronic acid functionality. The response of boronic acid-carrying thermosensitive copolymer against RNA was investigated in aqueous media in the pH range 4-9. In the acidic pH region, an increase was observed in the lower critical solution temperature (LCST) of the APBA-coupled thermosensitive copolymer with increasing RNA concentration. However, LCST decreased with increasing RNA concentration at both neutral and alkaline pH values. The LCST of the APBA-attached copolymer varied linearly with the RNA concentration at pH of 3, 4 and 7. (C) 2003 Society of Chemical Industry.