Prolific shoot regeneration from immature embryo explants of sainfoin (Onobrychis viciifolia Scop)

Ozcan S., Sevimay C. S., Yildiz M., SANCAK C., Ozgen M.

PLANT CELL REPORTS, sa.3-4, ss.200-203, 1996 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1007/s002990050206
  • Dergi Adı: PLANT CELL REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.200-203
  • Anahtar Kelimeler: immature cotyledon, immature embryo axis, Onobrychis viciifolia, adventitious shoot regeneration, PISUM-SATIVUM-L, PLANT-REGENERATION, SOMATIC EMBRYOGENESIS, COTYLEDONS, CULTURE, PEA, PROTOPLASTS
  • Ankara Üniversitesi Adresli: Evet

Özet

Immature cotyledons and embryo axes of sainfoin were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 6-benzylaminopurine (BAP) and alpha-naphthaleneacetic acid (NAA) to induce adventitious shoot regeneration. The highest frequency of shoot regeneration occurred following an initial callus growth on a MS medium containing 0.5 mg/l BAP and 2 mg/l NAA Immature embryo axes showed higher regeneration capacity than immature cotyledons, however, shoot elongation was best achieved on immature cotyledons. Regenerated shoots were excised and rooted in half strength MS medium with 1 mg/l indole-butyric acid (IBA) or 1 mg/l NAA. The rooted plantlets were finally transferred to compost.