In vitro micropropagation of the aquarium plant Ludwigia repens
Asia-Pacific Journal of Molecular Biology and Biotechnology, vol.12, no.1-2, pp.21-25, 2004 (Scopus)
- Publication Type: Article / Article
- Volume: 12 Issue: 1-2
- Publication Date: 2004
- Journal Name: Asia-Pacific Journal of Molecular Biology and Biotechnology
- Journal Indexes: Scopus
- Page Numbers: pp.21-25
- Keywords: 6-benzylaminopurine, And carry over effect, Ludwigia repens, Micropropagation, Thidiazuron (TDZ)
- Ankara University Affiliated: Yes
Abstract
Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and α-naphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm -3 TDZ and 0.1 mg dm-3 NAA. It was noted that TDZ or BAP had inhibitory effect on shoot elongation, which was overcome by subculturing shoots on half-strength MS media without growth regulators after 4 weeks of culture. This also served as rooting media. Rooted plantlets were finally transferred to aquariums containing fresh water with 100% adaptation.