Abiotic factors affecting <i>in vitro</i> pollen grain germination in sainfoin (<i>Onobrychis viciifolia</i> Scop.)


SANCAK C., Cocu S., Khawar K., Ozcan S.

PAKISTAN JOURNAL OF BOTANY, no.4, pp.463-471, 2003 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2003
  • Journal Name: PAKISTAN JOURNAL OF BOTANY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.463-471
  • Ankara University Affiliated: Yes

Abstract

A reliable in vitro method for germination of sainfoin pollen grains has been developed. Highest pollen germination rate of 96% with mean pollen tube length of 223.5 mum was obtained on medium containing of 100 g/l sucrose, 200 mg/l KNO3, 150 mg/l H3BO3, 150 mg/l MgSO4, 150 mg/l Ca(NO3)(2), 175 g/l PEG 4000 and stigma extract at pH 6.5.