A Novel PCR-RFLP Method for Detection of POR*28 Polymorphism and its Genotype/Allele Frequencies in a Turkish Population

Ozdemir F., DEMİRBÜGEN ÖZ M., SÜZEN H. S.

CURRENT DRUG METABOLISM, vol.20, no.10, pp.845-851, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 10
  • Publication Date: 2019
  • Doi Number: 10.2174/1389200220666190913121052
  • Journal Name: CURRENT DRUG METABOLISM
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.845-851
  • Keywords: CYP oxidoreductase, POR*28, A503V, Turkish, PCR-RFLP, pharmacogenetics, metabolism, OXIDOREDUCTASE GENETIC POLYMORPHISMS, HUMAN P450 OXIDOREDUCTASE, ACTIVITY IN-VIVO, CYTOCHROME-P450, CYP3A, TACROLIMUS, PHARMACOKINETICS, VARIANTS, IMPACT, CYP3A4-ASTERISK-22
  • Ankara University Affiliated: Yes

Abstract

Background: The Cytochrome P450 (CYP) enzymes are involved in the metabolism of many endogenous and exogenous substances. They need electrons for their activity. CYP mediated oxidation reactions require cytochrome oxidoreductase (POR) as an electron donor. A common genetic variation identified in the coding region of POR gene (POR*28) leads to an alteration in POR activity by causing amino acid change. The current study aimed to determine the allele and genotype frequencies of POR*28 in a healthy Turkish population by using a novel genotyping assay.