Antioxidant activity and phytochemical analysis of Alchemilla persica Rothm

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ERGENE B., BAHADIR ACIKARA Ö., BAKAR ATEŞ F., SALTAN İŞCAN H. G., Nebioǧlu S.

Ankara Universitesi Eczacilik Fakultesi Dergisi, vol.39, no.2, pp.145-154, 2010 (Scopus)

• Publication Type: Article / Article
• Volume: 39 Issue: 2
• Publication Date: 2010
• Doi Number: 10.1501/eczfak_0000000560
• Journal Name: Ankara Universitesi Eczacilik Fakultesi Dergisi
• Journal Indexes: Scopus
• Page Numbers: pp.145-154
• Keywords: Alchemilla persica, Antioxidant activity, DPPH, MDA, Rosaceae
• Ankara University Affiliated: Yes

Abstract

In present study, the extracts prepared using aerial parts and roots of Alchemilla persica Rothm. were evaluated for their antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and measurement of malondialdehyde (MDA) levels. HPLC analyses of the extracts were also performed using some phenolic acid and flavonoid standards. The hydro-methanolic extract of the aerial parts was found to possess significant antioxidant activity in both assays. IC50 values for the aerial parts and roots are determined as 0.055 M and 0.151 M respectively against DPPH radical. In TBARS assay, the MDA level of the aerial parts was found to be 5.9 nmol/ml, while 19.08 nmol/ml for the root extracts.