Akademik Veri Yönetim Sistemi
EMU International Pharmaceutical Sciences Congress (IPSC 2025), Famagusta, Kıbrıs (Kktc), 14 Mayıs 2025, ss.23, (Özet Bildiri)
Combination pharmaceutical products are widely
used in both human and veterinary medicine. Ensuring their quality requires accurate
and robust analytical methods that are preferably simple, low-cost and
environmentally friendly. However, the simultaneous quantitation of multiple
active compounds presents a challenge, especially in spectrophotometric
analysis due to overlapping spectra.
The aim of this study was to develop a fast,
accurate, and simple spectrophotometric method for the simultaneous
quantitation of clorsulon and ivermectin in a veterinary injectable formulation,
without requiring separation steps or complex sample preparation.
The injectable combination of clorsulon and
ivermectin is used in cattle to treat internal and external parasites. As their
UV spectra overlap extensively, their spectrophotometric analysis requires the
use of a suitable chemometric model. In this work, a partial least squares
(PLS) regression model was developed using a training set of 25 binary mixtures
of ivermectin (3.0–40.0 µg/mL) and clorsulon (2.5–36.0 µg/mL), designed via a
5² factorial design. The model was constructed using absorbance data in the
200–350 nm range (601×25 matrix) and corresponding concentration data (2×25
matrix). Cross-validation procedure indicated that three latent variables were
optimal for both analytes.
Analytical method validation was performed by
analyzing independent test mixtures in various concentrations, as well as intra-day,
inter-day and standard addition samples. Recovery results for ivermectin and
clorsulon in the test set were reported to be 100.11% and 101.38%,
respectively. After validation, the model was applied to commercial injectable
formulations, resulting in mean assay values of 10.25 mg/mL for ivermectin and
98.95 mg/mL for clorsulon, consistent with the labeled content.