Akademik Veri Yönetim Sistemi
Yükseköğretim Kurumları Destekli Proje, BAP Araştırma Projesi, 2015 - 2015
Objective:
Despite notable advancements in burn care, morbidity and mortality rates remain high, with progressive tissue loss typically occurring within the first 48 hours post-injury. While microneedling and topical retinyl palmitate are recognized for their regenerative effects in dermatology, their roles in acute burn wound management are not well defined. This study aimed to evaluate the early therapeutic impact of microneedling and retinyl palmitate on burn progression in a standardized rat model.
Materials
and Methods:
Twenty-four male Wistar albino rats were randomized into four groups (n=6): untreated control, microneedling, microneedling plus retinyl palmitate, and retinyl palmitate alone. Under general anesthesia, a standardized comb burn injury was inflicted on the dorsal skin. Microneedling was performed once, 30 minutes after injury in the respective groups. Retinyl palmitate was applied topically once daily for 28 days in the designated groups. Wound healing progression was evaluated macroscopically by monitoring wound size, body weight changes, and stasis zone survival. Histological assessment included Hematoxylin–Eosin, Masson’s Trichrome, Periodic Acid–Schiff, Verhoeff–Van Gieson staining, and immunohistochemistry for collagen types I and III. Parameters analyzed were re-epithelialization thickness, angiogenesis, inflammatory cell infiltration, and collagen deposition and alignment.
Results:
Microneedling significantly reduced wound area and improved preservation of the stasis zone (p<0.05). Histologically, microneedling induced near-complete re-epithelialization, minimal inflammatory infiltration, and organized collagen type I deposition resembling normal skin architecture. Retinyl palmitate monotherapy failed to improve healing and was associated with ongoing inflammation and delayed neovascularization. The combined treatment showed no advantage over microneedling alone.
Conclusion:
Microneedling applied in the acute phase of burn injury accelerates healing, preserves viable tissue, and promotes favorable collagen remodeling. These results highlight microneedling as a promising stand-alone intervention for early burn treatment and encourage further clinical research to refine therapeutic strategies