Farklı kavite dezenfektanlarının farklı etching modlarda uygulanan universal adezivin mikro-makaslama bağlanma dayanımına etkisi

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Thesis Type: Dental Specialty

Institution Of The Thesis: Ankara University, Diş Hekimliği Fakültesi, Klinik Bilimler Bölümü, Turkey

Approval Date: 2020

Thesis Language: Turkish

Student: TUĞÇE TARTICI

Supervisor: GÜRKAN GÜR

Open Archive Collection: AVESIS Open Access Collection

Abstract:

The aim of this study is to investigate the efficacy of different cavity disinfectants on the micro-shear bond strength of the universal adhesive applied in different etching modes as invitro. For this study, 80 freshly extracted sound human third molars were collected, cleaned and stored in distilled water. In each tooth, the occlusal enamel was removed by a cutting machine (Micracut 201, Metkon, Turkey) under water cooling to obtain flat dentin surfaces and then embedded in a self-curing acrylic resin. The resulting occlusal dentin surfaces were etched with 600-grit silicon carbide paper under water cooling to form a standard smear layer. 80 teeth, three different cavity disinfectant (OzoneDTA (APOZA, Taiwan), Chlorhexidine (CHX) (2% Bisco, Schaumberg), Super-oxide water (SPO) (Medilox, Turkey)), and such that any disinfection procedure APPLIED untreated control group 4 Single Bond Universal (SBU) (3M ESPE, St Paul, USA) was divided into 2 subgroups according to the application of the adhesive with etch-and-rinse and self-etch technique and randomly distributed in each main group. After the cavity disinfectant application, 1.5 mm long and 0.8 mm diameter polyethylene tubes (Unomedical, Conva-Tec Limited, England) were placed on the adhesive applied surfaces, and composite resin (Clearfil Majesty Flow A2, Kuraray Noritake Dental Inc.) was placed inside. Then the polyethylene tubes were removed with the help of a scalpel. 10,000 cycles were applied to 80 samples using an electronic thermal cycle device (Thermocycler the 1100, SD Mechatronik, Germany). Then Micro Shear bond strength (μSBS) was measured with a universal tester (Z010, Zwick, Ulm, Germany). As a result of the micro-shearing test, the areas where the composite ruptured from the tooth were evaluated with a stereomicroscope (M3Z, Leica Microsystems, Wetzlar, Germany) at 25 magnification for failure type analysis. Resin-dentin interfaces of one sample from each group were observed with TEM ((FEI Inspect F50 FEQ, Thermo Fisher Scientific Inc., Oregon, USA). According to the results of our study, the cavity disinfectant OzoneDTA, which showed the highest μSBS when applied with the universal adhesive etch-and-rinse technique, was found to be the CHX with the lowest μSBS. It was determined that SPO and control groups have the same values and have no effect on μSBS (p 0.05). According to our T test results, it was determined that the μSBSs for all cavity disinfectants in the etch-and-rinse technique were significantly higher than the μSBSs in the self-etch technique (p <0.05). It has been observed that the most common type of break at the adhesive-dentin interface is the adhesive type. The most common adhesive rupture type was observed when applied with universal adhesive selfetch technique. According to TEM findings, the acid applied to the dentin surface before the universal adhesive was used significantly improved the penetration of this adhesive into the dentin. Key words: Cavity disinfection, chlorhexidine gluconate, OzoneDTA, super-oxidized water, micro shear bond strength