Yüksek performanslı sıvı kromatografisi ve ilaç analizlerindeki uygulamaları

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Thesis Type: Postgraduate

Institution Of The Thesis: Ankara University, Sağlık Bilimleri Enstitüsü, ANALİTİK KİMYA ANABİLİM DALI, Turkey

Approval Date: 2006

Thesis Language: Turkish

Student: LALEZAR YENİLMEZ

Supervisor: SİBEL AYŞIL ÖZKAN

Open Archive Collection: AVESIS Open Access Collection

Abstract:

58SUMMARYHigh Performance Liquid Chromatography and its Application to DrugAnalysisHPLC is used extensively in the pharmaceutical industry and drug analysisdue to the availability of fully automated systems, and wide variety of columnstationary phases. This technique has excellent quantitative precision, accuracy, andbroad linear dynamic range. HPLC is used in drug discovery, pre-clinical, andclinical development and finished product analysis. Reverse phase high performanceliquid chromatography is the most widely used mode of modern liquidchromatography in drug analysis. C8 and C18 column are most commonly used as thestationary phases pharmaceutical analysis with UV detection. Other HPLC dedectorsused for drug analysis are fluorescence, electrochemical, refractive index, mass,infrared, evaporative light scattering. HPLC methods play a critical role in ensuringthe ruggedness and accuracy of methods used in the pharmaceutical industry. RapidHPLC methods have made significant progress in reduction of analysis times andmanagement of high throughput analysis. The functional types of HPLC methods,which are typically used in drug analysis are assay, impurity, stability and in-proces?Key Words: High performance Liquid chromatography, drug analysis, dedectors.