Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2012
Thesis Language: Turkish
Student: BURHANETTİN YALÇINKAYA
Supervisor: NUR MÜNEVVER PINAR
Abstract:Bu çalışmada Atmosferde en çok rastlanan mikrofungus cinslerine (Acremonium kiliense, Alternaria alternata, Aspergillus flavipes, Aspergillus niger, Aspergillus oryzae, Cladosporium herbarum, Cladosporium obtectum, Cylindrocladium floridanum, Penicillium chrysogenum), ait taksonların protein profillerinin karşılaştırılması, SDS-Page yöntemiyle yapılmıştır. Bu yönteme göre, Acremonium kliense, örneğinden SDS-Page yöntemi ile 19, Aspergillus flavipes örneğinden 17, Aspergillus niger’den 10, Aspergillus oryzae’den 13, Alternaria alternata’dan 31, Cladosporium herbarum’dan 25, Cladosporium obtectum’dan 16, Cylindrocladium floridanum’dan 14 ve Penicillium chrysogenum’dan 20 farklı protein bandı elde edilmiştir. Elde edilen verilere göre toplam 82 farklı protein bandı elde edilmiş, 24kda büyüklüğündeki protein bandının 7 taksonda (Acremonium kiliense, Aspergillus flavipes, Aspergillus niger, Aspergillus oryzae, Cladosporium obtectum, Cylindrocladium floridanum, Penicillium chrysogenum) ortak olduğu gözlemlenmiştir. Abstract In this study, the protein profile comparison of most commonly found microfungus genera taxa in the atmosphere (Acremonium kiliense, Alternaria alternata, Aspergillus flavipes, Aspergillus niger, Aspergillus oryzae, Cladosporium herbarum, Cladosporium obtectum, Cylindrocladium floridanum, Penicillium chrysogenum) was conducted using SDS-Page. According to this method, different protein bands for Acremonium kliense (19), Aspergillus flavipes (17), Aspergillus niger (10), Aspergillus oryzae (13), Alternaria alternata (31), Cladosporium herbarum (25), Cladosporium obtectum (16), Cylindrocladium floridanum (14) and Penicillium chrysogenum (20) were obtained. Based on data, 82 different protein bands were obtained and 24kda-sized protein band was determined to be common for 7 taxa (Acremonium kiliense, Aspergillus flavipes, Aspergillus niger, Aspergillus oryzae, Cladosporium obtectum, Cylindrocladium floridanum, Penicillium chrysogenum).