Hybrid fed-batch bioreactor operation design: control of substrate uptake enhances recombinant protein production in high-cell-density fermentations

ÇALIK P., Hoxha B., ÇALIK GARCİA G., Ozdamar T. H.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.93, no.11, pp.3326-3335, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 93 Issue: 11
  • Publication Date: 2018
  • Doi Number: 10.1002/jctb.5696
  • Journal Name: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3326-3335
  • Keywords: hybrid fed-batch, bioreactor operation, high-cell-density fermentation, substrate uptake, fed-batch, Pichia pastoris, GAP promoter, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE PROMOTER, METHANOL FEEDING STRATEGY, PICHIA-PASTORIS, GAP PROMOTER, EXPRESSION, SECRETION, MOLASSES, SYSTEMS
  • Ankara University Affiliated: Yes

Abstract

BACKGROUNDThe hybrid fed-batch bioreactor (HFBB) operation is a novel design method for high-cell-density fermentations. It employs fed-batch and batch operations, starting with fed-batch that in turn shifts to the next, and proceeds in successive cycles to enhance production and productivity by controlling the substrate uptake rate.