Determination of Some Endocrine Disrupting Chemicals with Selective and Sensitive Sensors

Project Supported by Other Official Institutions, 2021 - 2023

Project Type: Project Supported by Other Official Institutions
Begin Date: January 2021
End Date: January 2023

Project Abstract

Molecularly imprinted polymer (MIP) based electrochemical sensors were developed for the selective and sensitive determination of bisphenol A (BPA), bisphenol s (BPS) and bisphenol F (BPF). 5 mM [Fe(CN)₆]^3−/4–solution was used as the redox probe in electrochemical measurements. Non-imprinted polymer (NIP) was used to control the sensor performance.

During the preparation of the MIP based electrochemical sensor for BPA determination, electropolymerization was performed with functional monomer aniline. MIP(ANI)/GCE sensor has a linear range of 1x10^-15 M - 8x10^-15 M for standard solution and serum, limit of detection (LOD) and limit of quantification (LOQ) values at femtomolar level. The accuracy of the sensor was demonstrated by recovery studies in serum and plastic bottled water samples. Imprinting factor (IF) and interference studies proved the selectivity. This sensor is a highly sensitive and selective, low cost and reliable option for BPA analysis.

For the electrochemical analysis of BPS, a MIP-based sensor was prepared by photopolymerization using functional monomer N-methacryloyl-L-tyrosine. The linear range of MA-Tyr@MIP/GCE sensor was found as 1x10^-15 M - 1x10^-14 M, LOD and LOQ were calculated as 0.171 fM and 0.569 fM. Good recovery values obtained with serum and plastic bottled water samples. The sensor had high stability up to day 5. IF studies showed high selectivity.

For BPF analysis a porous MIP-based electrochemical sensor was prepared using L-Tryptophan, tetraethyl orthosilicate and cetyltrimethylammonium bromide. The linear range of MIP(TEOS:L-Trp)@GCE sensor for standard solution and serum sample was found as 1x10^-15 M - 1x10^-14 M. The LOD and LOQ showing the high sensitivity of the sensor were calculated as 0.291 fM and 0.971 fM. Good recovery% values obtained with plastic bottled water and serum samples. Interference and IF analyzes proved high selectivity. The sensor is stable up to 5 days.

The MIP-based sensors developed for the electrochemical analysis of BPA, BPS and BPF are more advantageous methods compared to existing methods.